Surface display of recombinant proteins on Bacillus thuringiensis spores.
نویسندگان
چکیده
The insecticidal protoxin from Bacillus thuringiensis has been shown to be a major component of the spore coat. We have developed a novel surface display system using B. thuringiensis spores in which the N-terminal portion of the protoxin is replaced with a heterologous protein. The expression vector with a sporulation-specific promoter was successfully used to display green fluorescent protein and a single-chain antibody (scFv) gene that encodes anti-4-ethoxymethylene-2-phenyl-2-oxazolin-5-one (anti-phOx) antibody. The spores that carry the anti-phOx antibody can bind to phOx specifically.
منابع مشابه
Laboratory Study of Microbial Insecticide Produced by the Bacteria Bacillus thuringiensis of Starch Factory Sewage for Biological Control of Larvae Culex pipiens
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ورودعنوان ژورنال:
- Applied and environmental microbiology
دوره 71 6 شماره
صفحات -
تاریخ انتشار 2005